The doctrine of the Immaculate Conception was proclaimed by Pius IV in 1854. The title of "Nuestra Señora De La Concepción Inmaculada" may be properly assigned to a variety of Marian figures. These all refer to the purity of Mary, although they may appear in a milieu of different poses and allegories.

Our Lady of the Immaculate Conception is shown here in two different representations. The first shows Mary as a young girl with a crown of roses, with her hands crossed over her heart, holding a dove, the symbol of the Holy Spirit, and staffs of roses and lilies to her chest, which represent her purity. She has a halo of stars around her head and clouds framing her.

The second version depicts Mary standing on top of a blue globe and/or a crescent moon, her hands folded in prayer and wearing a crown with the sun shining from behind her. Sometimes Mary is standing on a serpent which encircles the globe, symbolizing Mary as the second Eve, as Christ is often equated as the second Adam. Frequently there are two trees flanking her, probably a cypress and a palm, the cypress symbolizing death and the palm signifying triumph over sin and death. The halo of stars is sometimes transferred to the blue robe and Mary may be accompanied by angels or cherubs.

The subject of the Inmaculada is very common in retablo painting, although there is no particular patronage related to the image.